Targeting Constructs for Transgenic Model Generation
Recombineering (Red/ET recombination) has become the method of choice to engineer large replicons, such as large targeting constructs or bacterial artificial chromosomes (BACs). Since recombineering can be performed at any position in the DNA, the technology has opened up an unlimited number of possibilities for complex genetic modifications.
With over 20 years experiences and the know-how from over 1000 DNA engineering projects, Gen-H is the partner of choice for DNA modifications and targeting constructs for animal model generation.
Gen-H’s scientists have been involved in collaborative research projects and functional genomics programs funded by the European Commission, such as the “AgedBrainSYSBIO” which is a project with high impact on human public health. As part of this project, we successfully knocked in a 33 kb human fragment into the mouse ApoE locus.
Gen-H can assist you with broad expertise in generating optimized, complex targeting constructs for animal models.
Our services include:
Provided services
- Personal and customer-oriented project discussion by our scientists
- Customized construct design
- Direct contact partner over the period of the project
- Screening strategies for genotyping
What we offer:
Targeting constructs
- Knock-out targeting constructs
- Conditional knock-out targeting constructs
- Knock-in targeting constructs (e.g. reporter genes, CRE-ERT2, point mutations, tags)
- Conditional knock-in targeting constructs (induction of point mutation in a temporal- and tissue specific manner by Cre recombination)
- Humanized targeting constructs
BAC transgenes
- Removal of “passenger genes” from a BAC clone (“BAC shaving”)
- BAC fusions (“BAC stitchings”)
- Humanized BAC transgenes
- Insertions or deletions
Lentiviral constructs
- Individually and specifically engineered lentiviral constructs used for viral vector-based gene therapy
Examples
- Common schizophrenia risk variants are enriched in open chromatin regions of human glutamatergic Neurons; Mads E. Hauberg, Jordi Creus-Muncunill, Jaroslav Bendl, Alexey Kozlenkov, Biao Zeng, Chuhyon Corwin, Sarah Chowdhury, Harald Kranz, Yasmin L. Hurd, Michael Wegner, Anders D. Børglum, Stella Dracheva, Michelle E. Ehrlich, John F. Fullard and Panos Roussos; Nature Communications; 2020; 11: 5581
- Humanization of a large genome fragment using CRISPR/Cas9 and Recombineering; Martina Reiss and Harald Kranz; Biospektrum 07/2017
- BIN1 genetic risk factor for Alzheimer is sufficient to induce early structural tract alterations in entorhinal cortex-dentate gyrus pathway and related hippocampal multi-scale impairments; R Daudin, D Marechal, Q Wang, Y Abe, N Bourg, M Sartori, Y Loe-Mie, J Lipecka, C Guerrera, A McKenzie, B Potier, P Dutar, J Viard, A.M Lepagnol-Bestel, A Winkeler, V Hindié, MC Birling, L Lindner, C Chevalier, G Pavlovic, M Reis, H Kranz, G Dupuis, S Lévêque-Fort, J Diaz, E Davenas, D Dembele, J Laporte, C Thibault-Carpentier, B Malissen, J.C Rain, L Ciobanu, D Le Bihan, B Zhang, Y Herault and M Simonneau; bioRxiv; 2018: 437228
- Lentiviral-based approach for the validation of cancer therapeutic targets in vivo; Chiara Ambrogio, Patrick Stern, Claudio Scuoppo, Harald Kranz, Mariano Barbacid and David Santamaría; BioTechniques, 2014; 57: 179
- Modeling human disease in rodents by CRISPR/Cas9 genome Editing; Marie‑Christine Birling, Yann Herault and Guillaume Pavlovic, Mamm Genome; 2017; 28: 291
For more information please contact our service team in Heidelberg, Germany: contact (at) gen-h.de
Address
Gen-H Genetic Engineering Heidelberg GmbH
Im Neuenheimer Feld 584
69120 Heidelberg
Germany
Phone : +49 6221 1370811
Fax : +49 6221 1370829
contact (at) gen-h.de
Genetic Engineering Made in Germany
How to find us
Gen-H Genetic Engineering Heidelberg GmbH
Im Neuenheimer Feld 584
69120 Heidelberg