Our latest publication demonstrates a robust strategy for antibiotic-free maintenance of multiple plasmids using orthogonal conditional complementation.

You can read the article here.

Key highlights:

✅ Maintenance of two plasmids without antibiotics or resistance markers
✅ Functionality in non-supplemented media
✅ Flexible one- or two-plasmid configurations using orthogonal inducible promoters
✅ Productivity equivalent or superior to conventional antibiotic-selected systems

These results further establish conditional complementation as a versatile platform for plasmid maintenance and provide a practical, antibiotic-free solution for complex multi-plasmid biosynthetic pathways.