Available, ready-to-use adapetd E. coli strains

A wide range of well-known E. coli strains are already adapted to Gen-H´s antibiotic-free plasmid maintance system. All strains can be provided with your adapted plasmid of interest within 4 to 6 weeks.

Strain	Description
BL21	B strain for protein expression and precision fermentation Protease deficient
BL21-pgl	B strain for protein expression and precision fermentation Restored pentose phosphate pathway (pgl insertion) Elimination of B-strain dependent non-specific N-terminal protein gluconoylation Protease deficient
BL21(DE3)	B strain for T7-based protein expression and precision fermentation Protease deficient
T7E1	Gen-H´s engineered BL21(DE3) derivative with an inactivated DE3 prophage B strain for T7-based protein expression and precision fermentation Protease deficient
T7E2	Gen-H´s engineered BL21(DE3) derivative with an inactivated DE3 prophage B strain for T7-based protein expression and precision fermentation Protease deficient Restored pentose phosphate pathway (pgl insertion) Elimination of B-strain dependent non-specific N-terminal protein gluconoylation
W3110	K12 strain for protein expression and precision fermentation
MG1655	K12 strain for protein expression and precision fermentation
HSM174(DE3)	K12 strain for T7-based protein expression and precision fermentation
HSM174(T7)	Gen-H´s engineered HSM174(DE3) derivative with an inactivated DE3 prophage K12 strain for T7-based protein expression and precision fermentation
HS996	DH10β like K12 strain for plasmid-DNA production Resistant to phage T1 (fhuA modification)
HS996-pir116	DH10β like K12 strain for plasmid-DNA production Resistant to phage T1 (fhuA modification) Replication of R6K suicide plasmids
DH5α	K12 strain for plasmid-DNA production
W	Wildtype strain for protein production and precision fermentation Sucrose-metabolizing
Nissle1917	GRAS wildtype strain for protein production, precision fermentation and probiotic applications

For more information please contact our service team in Heidelberg, Germany: contact (at) gen-h.de

Address

Gen-H Genetic Engineering Heidelberg GmbH
Im Neuenheimer Feld 584
69120 Heidelberg
Germany

Phone : +49 6221 1370811
Fax : +49 6221 1370829
contact (at) gen-h.de

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Gen-H Genetic Engineering Heidelberg GmbH
Im Neuenheimer Feld 584
69120 Heidelberg

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+49 6221 13 70 811

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